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tev protease Results
More tev protease Results:
PRODUCTION AND PURIFICATION OF RECOMBINANT PENTACHLOROPHENOL 4-
... site is specific for tobacco etch virus (TEV) protease and it was located immediately after the factor ... In addition, a recombinant TEV protease containing a polyhistidine tag at the ...
http://www.nessling.fi/symposiot/Symposio2/wang.htm
Licensing Opportunity
A Mutant Of TEV Protease That Is Resistant To Autoinactivation. Description of Invention: This invention concerns a mutant of the tobacco etch virus (TEV) proteinase. ... Due to its high degree of sequence specificity, the TEV protease is valuable reagent for cleaving fusion proteins. However, the wild-type TEV protease also cleaves itself to yield a ...
http://ott.od.nih.gov/TextOnly/0-104799.htm
JBC -- Faber et al. 276 (39): 36501
... Additional applications of the TEV protease tostudy peroxisome biogenesis arediscussed ... co-synthesis of tev-containing substrateproteins and TEV protease (TEVp) derivatives, novel H ...
http://www.jbc.org/cgi/content/full/276/39/36501
Direct selection for sequences encoding proteases of known specificity.
Direct selection for sequences encoding proteases of known specificity. We have developed a simple genetic selection that could be used to isolate eukaryotic cDNAs encoding proteases that cleave ...
http://rdre1.yahoo.com/click?u=http://www.pubmedcentral.gov/articlerender.fcgi%253Fartid%253D51831%26y=02D93158EFB81F00%26i=487%26c=9107%26q=02%255ESSHPM%255BL7kzi%253Fompkz~lz6%26e=utf-8%26r=11%26d=nrm-en-us%26n=EB645H50UIH41GKE%26s=1%26t=%26m=40A30364%26x=0100A97F8D11C8AF
Non-Commercial Expression Vectors
... pSV281. T7lac. Kan. His. TEV protease. pSV278. T7lac. Kan ... pS219V allows expression of his-tagged TEV protease catalytic domain in E ...
http://www.structbio.vanderbilt.edu/wetlab/vectors.phtml
MCP -- Knuesel et al. 2 (11): 1225
... a cleavage site for thetobacco etch virus (TEV) protease, and the FLAG epitope, tothe N terminus ... peptide (CBP), separatedby a TEV protease cleavage site were fused to ...
http://www.mcponline.org/cgi/content/full/2/11/1225
SBNet - Conference posters 41 to 50
... Overproduction of an active soluble TEV protease fusion protein in Escherichia coli" ... A serious drawback has been that heterologous production of soluble TEV protease in E ...
http://alpha2.bmc.uu.se/~sbnet/conf02/posters_041_050.html
Orchestrating anaphase and mitotic exit: separase cleavage and localization of Slk19
... in metaphase, using tobacco etch virus (TEV) protease instead of separase, triggers separation of ... However, the spindle produced after TEV-protease-triggered anaphase seems weak and ...
http://www.nature.com/uidfinder/10.1038/ncb0901-771
JBC -- Phan et al. 277 (52): 50564
... It is unclear, however,exactly how TEV protease recognizes its substrates with such highselectivity. The crystal structures of two TEV protease mutants,inactive C151A and autolysis ...
http://www.jbc.org/cgi/content/full/277/52/50564
Protein Engineering, Design and Selection -- Abstracts: Kapust et al. 14 (12): 993
... However, a serious drawback of TEV proteaseis that it readily cleaves itself at a ... proportional to the concentration ofTEV protease, implying a bimolecular reaction mechanism ...
http://protein.oupjournals.org/cgi/content/abstract/14/12/993
Nucl. Acids. Res. -- Köhler 31 (4): e16
... which a substrate peptide of theTEV protease separates a myristoylation signal from a constitutively ... substrate (TPS) for the TEV protease (TEV-PR) separates a myristoylation signal ...
http://nar.oupjournals.org/cgi/content/full/31/4/e16
Purification and storage of TEV protease
Databases. ECCE. Maltose transporter. DNA sequences. Software. Dana's tools. Strategy. Gene expression in. E. coli. TEV NIa protease. as a tool. Search all files of this site ©. 2001 Webdesign by. Tilman Maier og Lars Jessen. 1. ... a French Press (1500 psi) Purify TEV protease by Ni-chromatography using standard protocol supplied by e ... Do not concentrate TEV protease too much (0.5 - 1 mg/ml) ...
http://www.cf.ac.uk/biosi/staff/ehrmann/tools/Purification.htm
Site-specific proteolysis of the Escherichia coli SecA protein in vivo.
Site-specific proteolysis of the Escherichia coli SecA protein in vivo. A seven-amino-acid cleavage site specific for tobacco etch virus (TEV) protease was introduced into SecA at two separate ...
http://rdre1.yahoo.com/click?u=http://www.pubmedcentral.gov/articlerender.fcgi%253Fartid%253D178040%26y=0299900126DF7077%26i=487%26c=9107%26q=02%255ESSHPM%255BL7kzi%253Fompkz~lz6%26e=utf-8%26r=15%26d=nrm-en-us%26n=EB645H50UIH41GKE%26s=1%26t=%26m=40A30364%26x=01C630BD98D36932
MCB -- Mueller and Jaehning 22 (7): 1971
... 50 ml of buffer A andthen equilibrated in TEV protease cleavage buffer (20 mM HEPES-KOH ... 200 U of TEV protease in 1 mlof TEV protease cleavage buffer ...
http://mcb.asm.org/cgi/content/full/22/7/1971
Structural Basis for the Substrate Specificity of Tobacco Etch Virus Protease*
... The crystal structures of two TEV protease mutants, inactive C151A and autolysis-resistant S219D, have now ... nants for TEV protease (7), whereas it is the P4 and P2 ...
http://mcl1.ncifcrf.gov/wlo_pubs/221.pdf
Purification and Isolation of GST Fusion Proteins
... which is a modified pET vector with a TEV protease cleavage site and a HIS tag seven amino ... Trying to do your TEV protease cleavage in the presence of inhibitors ...
http://xena.lsb.sbs.auckland.ac.nz/~gcard/Lisa/gst.purification.html
????? (PDF)
... is an enhanced form of Tobacco Etch Virus (TEV) protease that is highly site-specific, active, and more stable than native TEV. protease (1). AcTEV™. Protease recognizes the ...
http://www.lifetech.com/content/sfs/manuals/12575.pdf
SCOP: Protein: TEV protease (nucleat inclusion protein A, NIA) from Tobacco etch virus, TEV
... Protein: TEV protease (nucleat inclusion protein A, NIA) from Tobacco etch virus, TEV ... rather than forms a barrel. Protein: TEV protease (nucleat inclusion protein A, NIA) [82126 ...
http://pdb.weizmann.ac.il/scop/search.cgi?sunid=78234
TEV NIa protease homepage
Information about use of TEV protease for in vivo and in vitro proteolysis in Escherichia coli ... Tobacco Etch Virus (TEV) NIa protease is a very useful tool in molecular biology ... conserved Gln and Ser residues. TEV protease is a Cys protease and is thus inhibited by ...
http://www.cf.ac.uk/biosi/staff/ehrmann/tools/TEVprot.html
A generic protein purification method for protein complex characterization and proteome exploration
... After washing, the TEV protease is added to. release the bound material ... is required to remove the TEV protease as well. as traces of contaminants remaining after the ...
http://faculty.washington.edu/comai/TAP_natbiotech_99.pdf
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